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thermostable polymerases q5  (New England Biolabs)
99
New England Biolabs thermostable polymerases q5
a Synthetic template containing the UBP is PCR amplified with primers containing barcode (BC) sequences. b Raw fractions of B ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${f}_{B}$$\end{document} f B (+); blue) and S ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${f}_{S}$$\end{document} f S (−); gray) after PCR with Taq polymerase, including internal standards (BS or AT). PCR Step 2 represents a 1000× dilution of products from PCR Step 1. The mean ± SD of experimental replicates ( n = 3) is plotted with replicate values overlaid as points. c -Log 2 -transformed fraction B (B; blue), fraction S (S; gray), and average fraction ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\left\langle {{\rm{B}}},{{\rm{S}}}\right\rangle$$\end{document} B , S ; black), plotted as mean ± SD of experimental replicates ( n = 3). The average replication fidelity, \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varphi }_{\left\langle B,S\right\rangle }$$\end{document} φ B , S (95% CI) is calculated from the slope ( m ) of the regression line (dashed line) using the equation shown. d Fold-change in average UB fraction at a tested experimental versus reference condition ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${f}_{\left\langle B,S\right\rangle }/{f}_{\left\langle B,S\right\rangle }^{{ref}}$$\end{document} f B , S / f B , S r e f ) after a fixed value of theoretical cycles. Conditions include nucleotide concentration ([dNTP]; sky blue), polymerase (Pol.; purple), sequence context (Seq.; green), divalent cation (DV Cat.; orange), PCR supplements (Suppl.; gray), or pH (pH; blue). P -values are from a two-tailed Welch’s t -test ( n = 3 per group). e Average polymerase error rate ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varepsilon }_{\left\langle B,S\right\rangle }$$\end{document} ε B , S ) for <t>polymerases</t> with (purple) or without (gray) 3′ → 5′ exonuclease activity at two theoretical cycle values ( n = 3 per group). Mean values are plotted ± SD ( n = 6, replicates pooled). f \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varepsilon }_{\left\langle B,S\right\rangle }$$\end{document} ε B , S in 5 sequence contexts for Taq polymerase (gray) and <t>Q5</t> polymerase (green). WLS regression-derived estimates of \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varepsilon }_{\left\langle B,S\right\rangle }$$\end{document} ε B , S are plotted (95% CI; n = 3). Estimates of \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varepsilon }_{\left\langle B,S\right\rangle }$$\end{document} ε B , S from independent regression fits to each experimental replicate are overlaid as points. Asterisks denote statistical significance in a two-tailed z -test (** p < 0.01, * p < 0.05). g \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varepsilon }_{\left\langle B,S\right\rangle }$$\end{document} ε B , S for Taq polymerase at varying nucleotide concentration ratios of dBTP/dATP (gray), dSTP/dTTP (sky blue), or both (combined; white). Mean ± SD is plotted with experimental replicate values overlaid ( n = 3). h -Log 2 -transformed average UB fraction ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${f}_{\left\langle B,S\right\rangle }$$\end{document} f B , S ) in optimized PCR conditions. Mean ± SD is plotted with experimental replicate values overlaid ( n = 3). The raw data used to generate each plot and the exact p -values of statistical tests are tabulated in the Source Data file.
Thermostable Polymerases Q5, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/thermostable+dna+polymerase/Q5+HF+DNA+Pol/pmc13601594-293-0-17
Average 99 stars, based on 1 article reviews
thermostable polymerases q5 - by Bioz Stars, 2026-10
99/100 stars
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blend taq  (Toyobo)
96
Toyobo blend taq
a Synthetic template containing the UBP is PCR amplified with primers containing barcode (BC) sequences. b Raw fractions of B ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${f}_{B}$$\end{document} f B (+); blue) and S ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${f}_{S}$$\end{document} f S (−); gray) after PCR with Taq polymerase, including internal standards (BS or AT). PCR Step 2 represents a 1000× dilution of products from PCR Step 1. The mean ± SD of experimental replicates ( n = 3) is plotted with replicate values overlaid as points. c -Log 2 -transformed fraction B (B; blue), fraction S (S; gray), and average fraction ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\left\langle {{\rm{B}}},{{\rm{S}}}\right\rangle$$\end{document} B , S ; black), plotted as mean ± SD of experimental replicates ( n = 3). The average replication fidelity, \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varphi }_{\left\langle B,S\right\rangle }$$\end{document} φ B , S (95% CI) is calculated from the slope ( m ) of the regression line (dashed line) using the equation shown. d Fold-change in average UB fraction at a tested experimental versus reference condition ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${f}_{\left\langle B,S\right\rangle }/{f}_{\left\langle B,S\right\rangle }^{{ref}}$$\end{document} f B , S / f B , S r e f ) after a fixed value of theoretical cycles. Conditions include nucleotide concentration ([dNTP]; sky blue), polymerase (Pol.; purple), sequence context (Seq.; green), divalent cation (DV Cat.; orange), PCR supplements (Suppl.; gray), or pH (pH; blue). P -values are from a two-tailed Welch’s t -test ( n = 3 per group). e Average polymerase error rate ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varepsilon }_{\left\langle B,S\right\rangle }$$\end{document} ε B , S ) for <t>polymerases</t> with (purple) or without (gray) 3′ → 5′ exonuclease activity at two theoretical cycle values ( n = 3 per group). Mean values are plotted ± SD ( n = 6, replicates pooled). f \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varepsilon }_{\left\langle B,S\right\rangle }$$\end{document} ε B , S in 5 sequence contexts for Taq polymerase (gray) and <t>Q5</t> polymerase (green). WLS regression-derived estimates of \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varepsilon }_{\left\langle B,S\right\rangle }$$\end{document} ε B , S are plotted (95% CI; n = 3). Estimates of \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varepsilon }_{\left\langle B,S\right\rangle }$$\end{document} ε B , S from independent regression fits to each experimental replicate are overlaid as points. Asterisks denote statistical significance in a two-tailed z -test (** p < 0.01, * p < 0.05). g \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varepsilon }_{\left\langle B,S\right\rangle }$$\end{document} ε B , S for Taq polymerase at varying nucleotide concentration ratios of dBTP/dATP (gray), dSTP/dTTP (sky blue), or both (combined; white). Mean ± SD is plotted with experimental replicate values overlaid ( n = 3). h -Log 2 -transformed average UB fraction ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${f}_{\left\langle B,S\right\rangle }$$\end{document} f B , S ) in optimized PCR conditions. Mean ± SD is plotted with experimental replicate values overlaid ( n = 3). The raw data used to generate each plot and the exact p -values of statistical tests are tabulated in the Source Data file.
Blend Taq, supplied by Toyobo, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/thermostable+dna+polymerase/Blend+Taq/custom%40btq-101%4042142211
Average 96 stars, based on 1 article reviews
blend taq - by Bioz Stars, 2026-10
96/100 stars
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thermostable dna polymerases  (New England Biolabs)
99
New England Biolabs thermostable dna polymerases
a Synthetic template containing the UBP is PCR amplified with primers containing barcode (BC) sequences. b Raw fractions of B ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${f}_{B}$$\end{document} f B (+); blue) and S ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${f}_{S}$$\end{document} f S (−); gray) after PCR with Taq polymerase, including internal standards (BS or AT). PCR Step 2 represents a 1000× dilution of products from PCR Step 1. The mean ± SD of experimental replicates ( n = 3) is plotted with replicate values overlaid as points. c -Log 2 -transformed fraction B (B; blue), fraction S (S; gray), and average fraction ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\left\langle {{\rm{B}}},{{\rm{S}}}\right\rangle$$\end{document} B , S ; black), plotted as mean ± SD of experimental replicates ( n = 3). The average replication fidelity, \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varphi }_{\left\langle B,S\right\rangle }$$\end{document} φ B , S (95% CI) is calculated from the slope ( m ) of the regression line (dashed line) using the equation shown. d Fold-change in average UB fraction at a tested experimental versus reference condition ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${f}_{\left\langle B,S\right\rangle }/{f}_{\left\langle B,S\right\rangle }^{{ref}}$$\end{document} f B , S / f B , S r e f ) after a fixed value of theoretical cycles. Conditions include nucleotide concentration ([dNTP]; sky blue), polymerase (Pol.; purple), sequence context (Seq.; green), divalent cation (DV Cat.; orange), PCR supplements (Suppl.; gray), or pH (pH; blue). P -values are from a two-tailed Welch’s t -test ( n = 3 per group). e Average polymerase error rate ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varepsilon }_{\left\langle B,S\right\rangle }$$\end{document} ε B , S ) for <t>polymerases</t> with (purple) or without (gray) 3′ → 5′ exonuclease activity at two theoretical cycle values ( n = 3 per group). Mean values are plotted ± SD ( n = 6, replicates pooled). f \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varepsilon }_{\left\langle B,S\right\rangle }$$\end{document} ε B , S in 5 sequence contexts for Taq polymerase (gray) and <t>Q5</t> polymerase (green). WLS regression-derived estimates of \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varepsilon }_{\left\langle B,S\right\rangle }$$\end{document} ε B , S are plotted (95% CI; n = 3). Estimates of \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varepsilon }_{\left\langle B,S\right\rangle }$$\end{document} ε B , S from independent regression fits to each experimental replicate are overlaid as points. Asterisks denote statistical significance in a two-tailed z -test (** p < 0.01, * p < 0.05). g \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varepsilon }_{\left\langle B,S\right\rangle }$$\end{document} ε B , S for Taq polymerase at varying nucleotide concentration ratios of dBTP/dATP (gray), dSTP/dTTP (sky blue), or both (combined; white). Mean ± SD is plotted with experimental replicate values overlaid ( n = 3). h -Log 2 -transformed average UB fraction ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${f}_{\left\langle B,S\right\rangle }$$\end{document} f B , S ) in optimized PCR conditions. Mean ± SD is plotted with experimental replicate values overlaid ( n = 3). The raw data used to generate each plot and the exact p -values of statistical tests are tabulated in the Source Data file.
Thermostable Dna Polymerases, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/thermostable+dna+polymerase/T4+DNA+Polymerase/us12571798-1364-50-81
Average 99 stars, based on 1 article reviews
thermostable dna polymerases - by Bioz Stars, 2026-10
99/100 stars
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thermostable dna polymerase q5  (New England Biolabs)
99
New England Biolabs thermostable dna polymerase q5
a Synthetic template containing the UBP is PCR amplified with primers containing barcode (BC) sequences. b Raw fractions of B ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${f}_{B}$$\end{document} f B (+); blue) and S ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${f}_{S}$$\end{document} f S (−); gray) after PCR with Taq polymerase, including internal standards (BS or AT). PCR Step 2 represents a 1000× dilution of products from PCR Step 1. The mean ± SD of experimental replicates ( n = 3) is plotted with replicate values overlaid as points. c -Log 2 -transformed fraction B (B; blue), fraction S (S; gray), and average fraction ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\left\langle {{\rm{B}}},{{\rm{S}}}\right\rangle$$\end{document} B , S ; black), plotted as mean ± SD of experimental replicates ( n = 3). The average replication fidelity, \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varphi }_{\left\langle B,S\right\rangle }$$\end{document} φ B , S (95% CI) is calculated from the slope ( m ) of the regression line (dashed line) using the equation shown. d Fold-change in average UB fraction at a tested experimental versus reference condition ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${f}_{\left\langle B,S\right\rangle }/{f}_{\left\langle B,S\right\rangle }^{{ref}}$$\end{document} f B , S / f B , S r e f ) after a fixed value of theoretical cycles. Conditions include nucleotide concentration ([dNTP]; sky blue), polymerase (Pol.; purple), sequence context (Seq.; green), divalent cation (DV Cat.; orange), PCR supplements (Suppl.; gray), or pH (pH; blue). P -values are from a two-tailed Welch’s t -test ( n = 3 per group). e Average polymerase error rate ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varepsilon }_{\left\langle B,S\right\rangle }$$\end{document} ε B , S ) for <t>polymerases</t> with (purple) or without (gray) 3′ → 5′ exonuclease activity at two theoretical cycle values ( n = 3 per group). Mean values are plotted ± SD ( n = 6, replicates pooled). f \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varepsilon }_{\left\langle B,S\right\rangle }$$\end{document} ε B , S in 5 sequence contexts for Taq polymerase (gray) and <t>Q5</t> polymerase (green). WLS regression-derived estimates of \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varepsilon }_{\left\langle B,S\right\rangle }$$\end{document} ε B , S are plotted (95% CI; n = 3). Estimates of \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varepsilon }_{\left\langle B,S\right\rangle }$$\end{document} ε B , S from independent regression fits to each experimental replicate are overlaid as points. Asterisks denote statistical significance in a two-tailed z -test (** p < 0.01, * p < 0.05). g \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varepsilon }_{\left\langle B,S\right\rangle }$$\end{document} ε B , S for Taq polymerase at varying nucleotide concentration ratios of dBTP/dATP (gray), dSTP/dTTP (sky blue), or both (combined; white). Mean ± SD is plotted with experimental replicate values overlaid ( n = 3). h -Log 2 -transformed average UB fraction ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${f}_{\left\langle B,S\right\rangle }$$\end{document} f B , S ) in optimized PCR conditions. Mean ± SD is plotted with experimental replicate values overlaid ( n = 3). The raw data used to generate each plot and the exact p -values of statistical tests are tabulated in the Source Data file.
Thermostable Dna Polymerase Q5, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/thermostable+dna+polymerase/Q5+Hot+Start+High-Fidelity+DNA+Polymerase/pmc12828650-544-7-13
Average 99 stars, based on 1 article reviews
thermostable dna polymerase q5 - by Bioz Stars, 2026-10
99/100 stars
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thermostable t7 dna polymerase  (Amersham Pharmacia Biotech Ltd)
86
Amersham Pharmacia Biotech Ltd thermostable t7 dna polymerase
a Synthetic template containing the UBP is PCR amplified with primers containing barcode (BC) sequences. b Raw fractions of B ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${f}_{B}$$\end{document} f B (+); blue) and S ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${f}_{S}$$\end{document} f S (−); gray) after PCR with Taq polymerase, including internal standards (BS or AT). PCR Step 2 represents a 1000× dilution of products from PCR Step 1. The mean ± SD of experimental replicates ( n = 3) is plotted with replicate values overlaid as points. c -Log 2 -transformed fraction B (B; blue), fraction S (S; gray), and average fraction ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\left\langle {{\rm{B}}},{{\rm{S}}}\right\rangle$$\end{document} B , S ; black), plotted as mean ± SD of experimental replicates ( n = 3). The average replication fidelity, \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varphi }_{\left\langle B,S\right\rangle }$$\end{document} φ B , S (95% CI) is calculated from the slope ( m ) of the regression line (dashed line) using the equation shown. d Fold-change in average UB fraction at a tested experimental versus reference condition ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${f}_{\left\langle B,S\right\rangle }/{f}_{\left\langle B,S\right\rangle }^{{ref}}$$\end{document} f B , S / f B , S r e f ) after a fixed value of theoretical cycles. Conditions include nucleotide concentration ([dNTP]; sky blue), polymerase (Pol.; purple), sequence context (Seq.; green), divalent cation (DV Cat.; orange), PCR supplements (Suppl.; gray), or pH (pH; blue). P -values are from a two-tailed Welch’s t -test ( n = 3 per group). e Average polymerase error rate ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varepsilon }_{\left\langle B,S\right\rangle }$$\end{document} ε B , S ) for <t>polymerases</t> with (purple) or without (gray) 3′ → 5′ exonuclease activity at two theoretical cycle values ( n = 3 per group). Mean values are plotted ± SD ( n = 6, replicates pooled). f \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varepsilon }_{\left\langle B,S\right\rangle }$$\end{document} ε B , S in 5 sequence contexts for Taq polymerase (gray) and <t>Q5</t> polymerase (green). WLS regression-derived estimates of \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varepsilon }_{\left\langle B,S\right\rangle }$$\end{document} ε B , S are plotted (95% CI; n = 3). Estimates of \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varepsilon }_{\left\langle B,S\right\rangle }$$\end{document} ε B , S from independent regression fits to each experimental replicate are overlaid as points. Asterisks denote statistical significance in a two-tailed z -test (** p < 0.01, * p < 0.05). g \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varepsilon }_{\left\langle B,S\right\rangle }$$\end{document} ε B , S for Taq polymerase at varying nucleotide concentration ratios of dBTP/dATP (gray), dSTP/dTTP (sky blue), or both (combined; white). Mean ± SD is plotted with experimental replicate values overlaid ( n = 3). h -Log 2 -transformed average UB fraction ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${f}_{\left\langle B,S\right\rangle }$$\end{document} f B , S ) in optimized PCR conditions. Mean ± SD is plotted with experimental replicate values overlaid ( n = 3). The raw data used to generate each plot and the exact p -values of statistical tests are tabulated in the Source Data file.
Thermostable T7 Dna Polymerase, supplied by Amersham Pharmacia Biotech Ltd, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/thermostable+dna+polymerase/cycle+dyenamic+et+kit+sequencing+terminator/us12516375-619-18-22
Average 86 stars, based on 1 article reviews
thermostable t7 dna polymerase - by Bioz Stars, 2026-10
86/100 stars
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hs thermostable taq dna polymerase  (TaKaRa)
97
TaKaRa hs thermostable taq dna polymerase
a Synthetic template containing the UBP is PCR amplified with primers containing barcode (BC) sequences. b Raw fractions of B ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${f}_{B}$$\end{document} f B (+); blue) and S ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${f}_{S}$$\end{document} f S (−); gray) after PCR with Taq polymerase, including internal standards (BS or AT). PCR Step 2 represents a 1000× dilution of products from PCR Step 1. The mean ± SD of experimental replicates ( n = 3) is plotted with replicate values overlaid as points. c -Log 2 -transformed fraction B (B; blue), fraction S (S; gray), and average fraction ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\left\langle {{\rm{B}}},{{\rm{S}}}\right\rangle$$\end{document} B , S ; black), plotted as mean ± SD of experimental replicates ( n = 3). The average replication fidelity, \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varphi }_{\left\langle B,S\right\rangle }$$\end{document} φ B , S (95% CI) is calculated from the slope ( m ) of the regression line (dashed line) using the equation shown. d Fold-change in average UB fraction at a tested experimental versus reference condition ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${f}_{\left\langle B,S\right\rangle }/{f}_{\left\langle B,S\right\rangle }^{{ref}}$$\end{document} f B , S / f B , S r e f ) after a fixed value of theoretical cycles. Conditions include nucleotide concentration ([dNTP]; sky blue), polymerase (Pol.; purple), sequence context (Seq.; green), divalent cation (DV Cat.; orange), PCR supplements (Suppl.; gray), or pH (pH; blue). P -values are from a two-tailed Welch’s t -test ( n = 3 per group). e Average polymerase error rate ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varepsilon }_{\left\langle B,S\right\rangle }$$\end{document} ε B , S ) for <t>polymerases</t> with (purple) or without (gray) 3′ → 5′ exonuclease activity at two theoretical cycle values ( n = 3 per group). Mean values are plotted ± SD ( n = 6, replicates pooled). f \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varepsilon }_{\left\langle B,S\right\rangle }$$\end{document} ε B , S in 5 sequence contexts for Taq polymerase (gray) and <t>Q5</t> polymerase (green). WLS regression-derived estimates of \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varepsilon }_{\left\langle B,S\right\rangle }$$\end{document} ε B , S are plotted (95% CI; n = 3). Estimates of \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varepsilon }_{\left\langle B,S\right\rangle }$$\end{document} ε B , S from independent regression fits to each experimental replicate are overlaid as points. Asterisks denote statistical significance in a two-tailed z -test (** p < 0.01, * p < 0.05). g \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varepsilon }_{\left\langle B,S\right\rangle }$$\end{document} ε B , S for Taq polymerase at varying nucleotide concentration ratios of dBTP/dATP (gray), dSTP/dTTP (sky blue), or both (combined; white). Mean ± SD is plotted with experimental replicate values overlaid ( n = 3). h -Log 2 -transformed average UB fraction ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${f}_{\left\langle B,S\right\rangle }$$\end{document} f B , S ) in optimized PCR conditions. Mean ± SD is plotted with experimental replicate values overlaid ( n = 3). The raw data used to generate each plot and the exact p -values of statistical tests are tabulated in the Source Data file.
Hs Thermostable Taq Dna Polymerase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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thermostable pfu dna polymerase  (Thermo Fisher)
99
Thermo Fisher thermostable pfu dna polymerase
a Synthetic template containing the UBP is PCR amplified with primers containing barcode (BC) sequences. b Raw fractions of B ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${f}_{B}$$\end{document} f B (+); blue) and S ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${f}_{S}$$\end{document} f S (−); gray) after PCR with Taq polymerase, including internal standards (BS or AT). PCR Step 2 represents a 1000× dilution of products from PCR Step 1. The mean ± SD of experimental replicates ( n = 3) is plotted with replicate values overlaid as points. c -Log 2 -transformed fraction B (B; blue), fraction S (S; gray), and average fraction ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\left\langle {{\rm{B}}},{{\rm{S}}}\right\rangle$$\end{document} B , S ; black), plotted as mean ± SD of experimental replicates ( n = 3). The average replication fidelity, \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varphi }_{\left\langle B,S\right\rangle }$$\end{document} φ B , S (95% CI) is calculated from the slope ( m ) of the regression line (dashed line) using the equation shown. d Fold-change in average UB fraction at a tested experimental versus reference condition ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${f}_{\left\langle B,S\right\rangle }/{f}_{\left\langle B,S\right\rangle }^{{ref}}$$\end{document} f B , S / f B , S r e f ) after a fixed value of theoretical cycles. Conditions include nucleotide concentration ([dNTP]; sky blue), polymerase (Pol.; purple), sequence context (Seq.; green), divalent cation (DV Cat.; orange), PCR supplements (Suppl.; gray), or pH (pH; blue). P -values are from a two-tailed Welch’s t -test ( n = 3 per group). e Average polymerase error rate ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varepsilon }_{\left\langle B,S\right\rangle }$$\end{document} ε B , S ) for <t>polymerases</t> with (purple) or without (gray) 3′ → 5′ exonuclease activity at two theoretical cycle values ( n = 3 per group). Mean values are plotted ± SD ( n = 6, replicates pooled). f \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varepsilon }_{\left\langle B,S\right\rangle }$$\end{document} ε B , S in 5 sequence contexts for Taq polymerase (gray) and <t>Q5</t> polymerase (green). WLS regression-derived estimates of \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varepsilon }_{\left\langle B,S\right\rangle }$$\end{document} ε B , S are plotted (95% CI; n = 3). Estimates of \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varepsilon }_{\left\langle B,S\right\rangle }$$\end{document} ε B , S from independent regression fits to each experimental replicate are overlaid as points. Asterisks denote statistical significance in a two-tailed z -test (** p < 0.01, * p < 0.05). g \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varepsilon }_{\left\langle B,S\right\rangle }$$\end{document} ε B , S for Taq polymerase at varying nucleotide concentration ratios of dBTP/dATP (gray), dSTP/dTTP (sky blue), or both (combined; white). Mean ± SD is plotted with experimental replicate values overlaid ( n = 3). h -Log 2 -transformed average UB fraction ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${f}_{\left\langle B,S\right\rangle }$$\end{document} f B , S ) in optimized PCR conditions. Mean ± SD is plotted with experimental replicate values overlaid ( n = 3). The raw data used to generate each plot and the exact p -values of statistical tests are tabulated in the Source Data file.
Thermostable Pfu Dna Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/thermostable+dna+polymerase/DNA/10__3390_slash_nitrogen6040105-116-17-21
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thermostable pfu dna polymerase - by Bioz Stars, 2026-10
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thermostable dna polymerase nebnext q5 hot start hifi pcr master mix  (New England Biolabs)
97
New England Biolabs thermostable dna polymerase nebnext q5 hot start hifi pcr master mix
a Synthetic template containing the UBP is PCR amplified with primers containing barcode (BC) sequences. b Raw fractions of B ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${f}_{B}$$\end{document} f B (+); blue) and S ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${f}_{S}$$\end{document} f S (−); gray) after PCR with Taq polymerase, including internal standards (BS or AT). PCR Step 2 represents a 1000× dilution of products from PCR Step 1. The mean ± SD of experimental replicates ( n = 3) is plotted with replicate values overlaid as points. c -Log 2 -transformed fraction B (B; blue), fraction S (S; gray), and average fraction ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\left\langle {{\rm{B}}},{{\rm{S}}}\right\rangle$$\end{document} B , S ; black), plotted as mean ± SD of experimental replicates ( n = 3). The average replication fidelity, \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varphi }_{\left\langle B,S\right\rangle }$$\end{document} φ B , S (95% CI) is calculated from the slope ( m ) of the regression line (dashed line) using the equation shown. d Fold-change in average UB fraction at a tested experimental versus reference condition ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${f}_{\left\langle B,S\right\rangle }/{f}_{\left\langle B,S\right\rangle }^{{ref}}$$\end{document} f B , S / f B , S r e f ) after a fixed value of theoretical cycles. Conditions include nucleotide concentration ([dNTP]; sky blue), polymerase (Pol.; purple), sequence context (Seq.; green), divalent cation (DV Cat.; orange), PCR supplements (Suppl.; gray), or pH (pH; blue). P -values are from a two-tailed Welch’s t -test ( n = 3 per group). e Average polymerase error rate ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varepsilon }_{\left\langle B,S\right\rangle }$$\end{document} ε B , S ) for <t>polymerases</t> with (purple) or without (gray) 3′ → 5′ exonuclease activity at two theoretical cycle values ( n = 3 per group). Mean values are plotted ± SD ( n = 6, replicates pooled). f \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varepsilon }_{\left\langle B,S\right\rangle }$$\end{document} ε B , S in 5 sequence contexts for Taq polymerase (gray) and <t>Q5</t> polymerase (green). WLS regression-derived estimates of \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varepsilon }_{\left\langle B,S\right\rangle }$$\end{document} ε B , S are plotted (95% CI; n = 3). Estimates of \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varepsilon }_{\left\langle B,S\right\rangle }$$\end{document} ε B , S from independent regression fits to each experimental replicate are overlaid as points. Asterisks denote statistical significance in a two-tailed z -test (** p < 0.01, * p < 0.05). g \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varepsilon }_{\left\langle B,S\right\rangle }$$\end{document} ε B , S for Taq polymerase at varying nucleotide concentration ratios of dBTP/dATP (gray), dSTP/dTTP (sky blue), or both (combined; white). Mean ± SD is plotted with experimental replicate values overlaid ( n = 3). h -Log 2 -transformed average UB fraction ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${f}_{\left\langle B,S\right\rangle }$$\end{document} f B , S ) in optimized PCR conditions. Mean ± SD is plotted with experimental replicate values overlaid ( n = 3). The raw data used to generate each plot and the exact p -values of statistical tests are tabulated in the Source Data file.
Thermostable Dna Polymerase Nebnext Q5 Hot Start Hifi Pcr Master Mix, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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a Synthetic template containing the UBP is PCR amplified with primers containing barcode (BC) sequences. b Raw fractions of B ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${f}_{B}$$\end{document} f B (+); blue) and S ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${f}_{S}$$\end{document} f S (−); gray) after PCR with Taq polymerase, including internal standards (BS or AT). PCR Step 2 represents a 1000× dilution of products from PCR Step 1. The mean ± SD of experimental replicates ( n = 3) is plotted with replicate values overlaid as points. c -Log 2 -transformed fraction B (B; blue), fraction S (S; gray), and average fraction ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\left\langle {{\rm{B}}},{{\rm{S}}}\right\rangle$$\end{document} B , S ; black), plotted as mean ± SD of experimental replicates ( n = 3). The average replication fidelity, \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varphi }_{\left\langle B,S\right\rangle }$$\end{document} φ B , S (95% CI) is calculated from the slope ( m ) of the regression line (dashed line) using the equation shown. d Fold-change in average UB fraction at a tested experimental versus reference condition ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${f}_{\left\langle B,S\right\rangle }/{f}_{\left\langle B,S\right\rangle }^{{ref}}$$\end{document} f B , S / f B , S r e f ) after a fixed value of theoretical cycles. Conditions include nucleotide concentration ([dNTP]; sky blue), polymerase (Pol.; purple), sequence context (Seq.; green), divalent cation (DV Cat.; orange), PCR supplements (Suppl.; gray), or pH (pH; blue). P -values are from a two-tailed Welch’s t -test ( n = 3 per group). e Average polymerase error rate ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varepsilon }_{\left\langle B,S\right\rangle }$$\end{document} ε B , S ) for polymerases with (purple) or without (gray) 3′ → 5′ exonuclease activity at two theoretical cycle values ( n = 3 per group). Mean values are plotted ± SD ( n = 6, replicates pooled). f \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varepsilon }_{\left\langle B,S\right\rangle }$$\end{document} ε B , S in 5 sequence contexts for Taq polymerase (gray) and Q5 polymerase (green). WLS regression-derived estimates of \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varepsilon }_{\left\langle B,S\right\rangle }$$\end{document} ε B , S are plotted (95% CI; n = 3). Estimates of \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varepsilon }_{\left\langle B,S\right\rangle }$$\end{document} ε B , S from independent regression fits to each experimental replicate are overlaid as points. Asterisks denote statistical significance in a two-tailed z -test (** p < 0.01, * p < 0.05). g \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varepsilon }_{\left\langle B,S\right\rangle }$$\end{document} ε B , S for Taq polymerase at varying nucleotide concentration ratios of dBTP/dATP (gray), dSTP/dTTP (sky blue), or both (combined; white). Mean ± SD is plotted with experimental replicate values overlaid ( n = 3). h -Log 2 -transformed average UB fraction ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${f}_{\left\langle B,S\right\rangle }$$\end{document} f B , S ) in optimized PCR conditions. Mean ± SD is plotted with experimental replicate values overlaid ( n = 3). The raw data used to generate each plot and the exact p -values of statistical tests are tabulated in the Source Data file.

Journal: Nature Communications

Article Title: Quantifying replication fidelity of unnatural base pairs using nanopore sequencing

doi: 10.1038/s41467-026-76964-9

Figure Lengend Snippet: a Synthetic template containing the UBP is PCR amplified with primers containing barcode (BC) sequences. b Raw fractions of B ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${f}_{B}$$\end{document} f B (+); blue) and S ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${f}_{S}$$\end{document} f S (−); gray) after PCR with Taq polymerase, including internal standards (BS or AT). PCR Step 2 represents a 1000× dilution of products from PCR Step 1. The mean ± SD of experimental replicates ( n = 3) is plotted with replicate values overlaid as points. c -Log 2 -transformed fraction B (B; blue), fraction S (S; gray), and average fraction ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\left\langle {{\rm{B}}},{{\rm{S}}}\right\rangle$$\end{document} B , S ; black), plotted as mean ± SD of experimental replicates ( n = 3). The average replication fidelity, \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varphi }_{\left\langle B,S\right\rangle }$$\end{document} φ B , S (95% CI) is calculated from the slope ( m ) of the regression line (dashed line) using the equation shown. d Fold-change in average UB fraction at a tested experimental versus reference condition ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${f}_{\left\langle B,S\right\rangle }/{f}_{\left\langle B,S\right\rangle }^{{ref}}$$\end{document} f B , S / f B , S r e f ) after a fixed value of theoretical cycles. Conditions include nucleotide concentration ([dNTP]; sky blue), polymerase (Pol.; purple), sequence context (Seq.; green), divalent cation (DV Cat.; orange), PCR supplements (Suppl.; gray), or pH (pH; blue). P -values are from a two-tailed Welch’s t -test ( n = 3 per group). e Average polymerase error rate ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varepsilon }_{\left\langle B,S\right\rangle }$$\end{document} ε B , S ) for polymerases with (purple) or without (gray) 3′ → 5′ exonuclease activity at two theoretical cycle values ( n = 3 per group). Mean values are plotted ± SD ( n = 6, replicates pooled). f \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varepsilon }_{\left\langle B,S\right\rangle }$$\end{document} ε B , S in 5 sequence contexts for Taq polymerase (gray) and Q5 polymerase (green). WLS regression-derived estimates of \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varepsilon }_{\left\langle B,S\right\rangle }$$\end{document} ε B , S are plotted (95% CI; n = 3). Estimates of \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varepsilon }_{\left\langle B,S\right\rangle }$$\end{document} ε B , S from independent regression fits to each experimental replicate are overlaid as points. Asterisks denote statistical significance in a two-tailed z -test (** p < 0.01, * p < 0.05). g \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${\varepsilon }_{\left\langle B,S\right\rangle }$$\end{document} ε B , S for Taq polymerase at varying nucleotide concentration ratios of dBTP/dATP (gray), dSTP/dTTP (sky blue), or both (combined; white). Mean ± SD is plotted with experimental replicate values overlaid ( n = 3). h -Log 2 -transformed average UB fraction ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${f}_{\left\langle B,S\right\rangle }$$\end{document} f B , S ) in optimized PCR conditions. Mean ± SD is plotted with experimental replicate values overlaid ( n = 3). The raw data used to generate each plot and the exact p -values of statistical tests are tabulated in the Source Data file.

Article Snippet: Thermostable polymerases Q5 (M0491L), Phusion (M0530S), DeepVent (M0258S), DeepVent (exo-) (M0259S), and OneTaq (M0480S) were purchased from New England Biolabs (Ipswich, MA).

Techniques: Amplification, Transformation Assay, Concentration Assay, Sequencing, Two Tailed Test, Activity Assay, Derivative Assay